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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDKL1 Antibody (clone 1F12) IHC‑plus™ LS‑B6189
Note: This antibody replaces LS-C133577
CDKL1 antibody LS-B6189 is an unconjugated mouse monoclonal antibody to human CDKL1. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6189-50
50 µg (1 mg/ml)
Unavailable

Popular CDKL1 Products

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-CDKL1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4473 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-CDKL1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6188 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Human, Prostate: Formalin-Fixed Paraffin-Embedded (FFPE)
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

CDKL1 antibody LS-B6189 is an unconjugated mouse monoclonal antibody to human CDKL1. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CDKL1
CDKL1 product is a member of a large family of CDC2-related serine/threonine protein kinases. It accumulates primarily in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. Q00532 X66358 NP_004187.2

CDKL1 Antibody, CDC2-related kinase 1 Antibody, KKIALRE Antibody, p42 Antibody, Protein kinase p42 KKIALRE Antibody, Cyclin-dependent kinase-like 1 Antibody


Specifications

Target
Human CDKL1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [1F12]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDKL1 antibody was raised against cDKL1 (NP_004187, 259 a.a. ~ 358 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CDKL1
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot with the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4.
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6189 concentration 5 ug/ml.
Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6189 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CDKL1 on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to CDKL1 on HeLa cell. [antibody concentration 10 ug/ml].

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CDKL1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CDKL1 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6189 concentration 5 ug/ml.
Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6189 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CDKL1 on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to CDKL1 on HeLa cell. [antibody concentration 10 ug/ml].

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CDKL1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CDKL1 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6189 concentration 5 ug/ml.
Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6189 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CDKL1 on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to CDKL1 on HeLa cell. [antibody concentration 10 ug/ml].

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CDKL1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CDKL1 is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6189 concentration 5 ug/ml.
Anti-CDKL1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6189 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence of monoclonal antibody to CDKL1 on HeLa cell. [antibody concentration 10 ug/ml].
Immunofluorescence of monoclonal antibody to CDKL1 on HeLa cell. [antibody concentration 10 ug/ml].

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CDKL1 is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CDKL1 is approximately 0.03 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/22/2018

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