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Anti-CDH6 / K Cadherin Antibody (Extracellular Domain) LS-C35232

Ordering

Wt. Vol. Conc. Price
100 µg 500 µl 0.2 mg/ml $650
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular CDH6 / K Cadherin Antibodies

Anti-CDH6 / K Cadherin Antibody (clone 2B6) LS-C26845
Mouse Monoclonal [clone 2B6] (IgG1)
Human, Rat
IHC - Frozen, ICC, Western blot
Unconjugated
Anti-CDH6 / K Cadherin Antibody (Extracellular Domain) LS-C35232
Sheep Polyclonal (IgG) to Human CDH6 / K Cadherin
Human
ICC, Western blot, Flow Cytometry, ELISA
Unconjugated
Anti-CDH6 / K Cadherin Antibody (clone 2B6) IHC-plus™ LS-B6726
Mouse Monoclonal [clone 2B6] (IgG1) to Human CDH6 / K Cadherin
Human, Rat
IHC - Paraffin, IHC - Frozen, ICC, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human CDH6 / K Cadherin
Human
ICC, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human CDH6 / K Cadherin
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (10 µg/ml)
  • Western blot
  • Flow Cytometry (50 µg/ml)
  • ELISA (0.5 - 1 µg/ml)

Specificity and Use

CDH6 / K Cadherin antibody was raised against nS0-derived rhCadherin-6 extracellular domain.
Extracellular Domain
Recognizes human Cadherin-6. In direct ELISAs and Western Blot, shows less than 5% cross-reactivity with rhCadherin-8, rhCadherin-11, and rhCadherin-12.
Suitable for use in Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. Western Blot: 0.1 - 0.2 ug/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit is approximately 10 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 ug/mL to detect human Cadherin-6 in MG63 human osteosarcoma cells cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. Flow Cytometry: 50 ug/ml Add 10 ul of the diluted solution to 1-2.5x10^5 MG63 human osteosarcoma cells. This antibody has been tested on MG63 human osteosarcoma cells. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200uL. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as anti-sheep lgG conjugated to a fluorochrome. Direct ELISA: 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human Cadherin-6. The detection limit for rhCadherin-6 is approximately 1 ng/well.

Packaging

Lyophilized from PBS, 5% trehalose.
500 µl Sterile PBS.
Long term: -20°C; Short term: -20°C
For research use only.

About CDH6 / K Cadherin

P55285 NM_004932 P55285

CDH6 Antibody, K Cadherin Antibody, K-cadherin Antibody, CAD6 Antibody, Cadherin-6 Antibody, Fetal kidney cadherin Antibody, KCAD Antibody, Kidney cadherin Antibody

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

Requested From: United States
Date Requested: 12/3/2016

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