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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDH2 / N Cadherin Antibody (clone 5D5) IHC‑plus™ LS‑B4160
Note: This antibody replaces LS-C109156
N Cadherin antibody LS-B4160 is an unconjugated mouse monoclonal antibody to human N Cadherin (CDH2). Validated for ELISA, Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4160-50
50 µl
Unavailable

Popular CDH2 / N Cadherin Products

Antibody
Species: Human, Mouse, Bovine, Chicken
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
Staining of the Hela cell line with Biotin Mouse IgG1, K isotype control (blue histogram) or Biotin anti-human CD325 (N-cadherin ) (purple histogram) followed by Sav-PE. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
Staining of HeLa cells with PE Mouse IgG1, K isotype control (blue histogram) or PE anti-human CD325 (8C11) (purple histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-CDH2 mouse monoclonal antibody.
Species: Human, Monkey, Mouse, Dog
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

Product Description

N Cadherin antibody LS-B4160 is an unconjugated mouse monoclonal antibody to human N Cadherin (CDH2). Validated for ELISA, Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CDH2 / N Cadherin
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. P19022 NM_001792 NP_001783.2

CDH2 Antibody, CDw325 Antibody, CDHN Antibody, Cadherin-2 Antibody, CD325 Antibody, CD325 antigen Antibody, N Cadherin Antibody, N-cadherin Antibody, NCAD Antibody, Neural cadherin Antibody, N-cadherin 1 Antibody, Neural-cadherin Antibody


Specifications

Target
Human CDH2 / N Cadherin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [5D5]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of human CDH2 expressed in E. coli.
Specificity
Human, mouse and rat
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CDH2 / N Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.
Anti-CDH2 / N Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.

Immunohistochemistry

Anti-CDH2 / N Cadherin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.
Anti-CDH2 / N Cadherin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.

Immunofluorescence

Immunofluorescence of A431 cells using CDH2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of A431 cells using CDH2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using CDH2 mouse monoclonal antibody against A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.
Western blot using CDH2 mouse monoclonal antibody against A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.

Immunohistochemistry

Anti-CDH2 / N Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.
Anti-CDH2 / N Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.

Immunohistochemistry

Anti-CDH2 / N Cadherin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.
Anti-CDH2 / N Cadherin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.

Immunofluorescence

Immunofluorescence of A431 cells using CDH2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of A431 cells using CDH2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using CDH2 mouse monoclonal antibody against A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.
Western blot using CDH2 mouse monoclonal antibody against A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.

Immunohistochemistry

Anti-CDH2 / N Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.
Anti-CDH2 / N Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.

Immunohistochemistry

Anti-CDH2 / N Cadherin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.
Anti-CDH2 / N Cadherin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.

Immunofluorescence

Immunofluorescence of A431 cells using CDH2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of A431 cells using CDH2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using CDH2 mouse monoclonal antibody against A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.
Western blot using CDH2 mouse monoclonal antibody against A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.

Immunohistochemistry

Anti-CDH2 / N Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.
Anti-CDH2 / N Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.

Immunohistochemistry

Anti-CDH2 / N Cadherin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.
Anti-CDH2 / N Cadherin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4160 dilution 1:200.

Immunofluorescence

Immunofluorescence of A431 cells using CDH2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence of A431 cells using CDH2 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot using CDH2 mouse monoclonal antibody against A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.
Western blot using CDH2 mouse monoclonal antibody against A431 (1), NIH/3T3 (2), HeLa (3), C6 (4) and LNCap (5) cell lysate.


Requested From: 
Date Requested: 6/20/2018

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