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Anti-CDC25A Antibody LS-C43053

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $350
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CDC25A Antibodies

Anti-CDC25A Antibody (Ser75) IHC-plus™ LS-B1463
Rabbit Polyclonal (IgG) to Human CDC25A
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CDC25A Antibody LS-C43053
Rabbit Polyclonal (IgG) to Human CDC25A
Human
Western blot, Immunoprecipitation
Unconjugated
Western blot Image
Anti-CDC25A Antibody (phospho-Thr507) LS-C97430
Rabbit Polyclonal to Human CDC25A
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human CDC25A
Human
Western blot, Immunoprecipitation
Unconjugated

Details

Human CDC25A
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • Western blot (1:1000 - 1:10000)
  • Immunoprecipitation

Specificity and Use

CDC25A antibody was raised against synthetic peptide corresponding to a region of human CDC25a encoded within exon 9.
Recognizes Cdc25A (Cell division cycle 25A), a dual specificity protein phosphatase which is involved in the regulation of the cell cycle. Cdc25A is required for progression from G1 to the S phase of the cell cycle, although the protein remains active after the S phase. The activity of Cdc25A is regulated by reversible phosphorylation. Cdc25A is degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. Cdc25A is overexpressed by a wide variety of human cancers. The epitope recognized bymaps to a region between residues 175 and 225 of human Cdc25A using the numbering given in NP_001780.2.
Western Blot: After immunoprecipitation followed by Western blotting, LS-C43053 detects a band of approximately 68 kD in HeLa cell lysates.

Packaging

Tris-citrate/phosphate buffer, 0.1% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About CDC25A

P30304 NM_001789 NP_001780.2

CDC25A Antibody, CDC25A2-CAG isoform Antibody, Cell division cycle 25A Antibody, CDC25A phosphatase Antibody, CDC25Hu1 Antibody, M-phase inducer phosphatase 1 Antibody, CDC25A2 Antibody

CDC25A, a CDC25 dual specificity protein phosphatase, has been shown to activate the cyclin-dependent kinases at different points of the cell cycle. CDC25A has been shown to affect S phase entry and possibly the initiation of mitosis. CDC25A dephosphorylates cyclin dependent kinases, including Cdk2, Cdk4, as well as non cyclin proteins such as the homeodomain transcription factor cut, the proto-oncogene Raf-1.

Western blot

Western blot
Detection of human Cdc25 in nuclear extracts from HeLa cells following immunoprecipitation and Western blotting using Rabbit anti-Human Cdc25 (LS-C43053).
 

Requested From: United States
Date Requested: 12/5/2016

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