Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [O13]
IHC - Paraffin (1:10 - 1:40)
IHC - Frozen (1:10 - 1:40)
Specificity and Use
CD99 antibody was raised against meWo human melanoma cell line.
Recognizes the human CD99 cell surface antigen, a 32kD glycoprotein expressed by leucocytes and strongly on the majority (>95%) of Ewings sarcomas. The antigen is also expressed by some primitive neuroectodermal tumors and peripheral neuroepitheliomas.
Immunohistology: This product requires antigen retrieval using heat treatment methods prior to staining of paraffin sections.
PBS, 0.09% sodium azide, 1% BSA.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99.