Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Tissue culture supernatant
IHC - Paraffin (1:25 - 1:50)
IHC - Frozen (1:25 - 1:50)
Specificity and Use
CD9 antibody was raised against recombinant protein encoding the major extracellular loop of human CD9.
Recognizes CD9 at 24-27kD. Species Reactivity: Human.
Immunohistochemistry (frozen & formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min., followed by cooling at RT for 20 min. Immunohistochemistry: 1:25 to 1:50.
Concentrated tissue culture supernatant in 15 mM sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.