Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone KMC8] (IgG2a,k) to Mouse CD9
Western blot, Flow Cytometry
Mouse (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [KMC8]
Specificity and Use
CD9 antibody was raised against mouse CD9
This KMC8 (KMC8) antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.
Staining of C57Bl/6 bone marrow cells with 0.5 ug of Purified Rat IgG2a isotype control (open histogram) or 0.5 ug of Purified anti-mouse CD9 (KMC8) (colored histogram) followed by PE Anti-Rat IgG (LS-C106534). Cells in the large scatter population were used for analysis.
Requested From: United States
Date Requested: 1/20/2017