Mouse CD8A / CD8 Alpha

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

53-6.72

Phycoerythrin, Cy7. Also available Unconjugated or conjugated with Biotin, FITC, RPE, APC.

Protein G purified

Unmodified

Mouse thymocytes/splenocytes.

Mouse CD8.

• Flow Cytometry
• ELISA
• Applications tested for the base form of this product only

Reagent was tested for binding to ACKlysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 ul of 300 ug/ml Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-Mouse CD8/PE-Cy7 at a 1:50 dilution (5 ug/ml). They were then washed three times, fixed and analyzed by FACS. An 8.3% sub population of the cells stained positive with a mean shift of 1.4 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-mouse CD8 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA

Store at 4°C. Do not freeze. Protect from light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01732 NCBI: NM_001768 NP_001759.3