Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Rat Monoclonal (IgG2a,k) to Mouse CD86
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Mouse (tested or 100% immunogen sequence identity)
IHC - Frozen
Specificity and Use
CD86 antibody was raised against lPS-activated mouse B cells.
Recognizes mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4.
Flow cytometry. Immunohistochemistry (frozen sections). Immunoprecipitation. In vivo and in vitro blocking assays. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS. No preservative added.
Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. It is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell.