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Anti-CD83 Antibody (aa22-134) LS-C150174

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD83 Antibodies

Anti-CD83 Antibody (clone HB15a) LS-C5618
Mouse Monoclonal [clone HB15a] (IgG2b,k) to Human CD83
Human
IHC, Immunofluorescence, Flow Cytometry
Unconjugated
Anti-CD83 Antibody (aa103-152) IHC-plus™ LS-B11636
Rabbit Polyclonal to Human CD83
Human, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Mouse CD83
Mouse
ICC, Western blot, Flow Cytometry
Unconjugated

Details

Mouse CD83
Goat
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Flow Cytometry

Specificity and Use

CD83 antibody was raised against mouse myeloma cell line NS0-derived recombinant mouse CD83 Met22-Ala134 Accession # O88324.
aa22-134
Detects mouse CD83 in direct ELISAs and Western blots.
Western Blot, 0.1 µg/ml, Recombinant Mouse CD83 Fc Chimera. Flow Cytometry, 2.5 µg/106 cells, LPS-treated mature mouse dendritic cells. Immunocytochemistry, 5-15 µg/ml, Adhesion Blockade, The adhesion of human monocyte-derived dendritic cells (5 x 104 cells/well) to immobilized Recombinant Mouse. CD83 Fc Chimera was maximally inhibited (80-100%) by 40 µg/ml of the antibody. 5., Zhou, L-J et. al 1 (99.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About CD83

Q01151 NM_004233 NP_004224.1

CD83 Antibody, BL11 Antibody, Cell surface protein HB15 Antibody, CD83 antigen Antibody, CD83 molecule Antibody, Cell-surface glycoprotein Antibody, HCD83 Antibody, B-cell activation protein Antibody, HB15 Antibody

CD83 is a member of the Ig superfamily. It contains an extracellular V-type domain, a single transmembrane domain and a 40 residue cytoplasmic tail. CD83 is used as a marker for mature, antigen presenting dendritic cells that are capable of generating tumor specific T cell immunity. CD83 is expressed by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centers.

Immunocytochemistry

Immunocytochemistry
CD83 in Mouse Dendritic Cells. CD83 was detected in immersion fixed LPS-stimulated mouse dendritic cells using Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Immunocytochemistry

Immunocytochemistry
CD83 in Mouse Splenocytes. CD83 was detected in immersion fixed mouse splenocytes using 10 ug/ml Goat Anti-Mouse CD83 Antigen Affinity-purified Polyclonal Antibody for 3 hours at room temperature. Cells were stained with the NorthernLights 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue).

Requested From: United States
Date Requested: 12/5/2016

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