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Anti-CD71 / Transferrin Receptor Antibody (clone YTA74.4) LS-C43241

Note: This antibody replaces LS-C57161, LS-C43242

Catalog	Size	Price
LS-C43241-250	250 µg (1 mg/ml)	Unavailable

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Antibody:	Rat Monoclonal [clone YTA74.4] (IgG2a) to Mouse CD71 / Transferrin Receptor
Reactivity:	Mouse
Application:	IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Mouse CD71 / Transferrin Receptor
Host:	Rat
Reactivity:	Mouse (tested or 100% immunogen sequence identity)
Clonality:	IgG2a Monoclonal [YTA74.4]
Conjugations:	Unconjugated. Also available conjugated with FITC, RPE.
Purification:	Protein G purified
Modifications:	Unmodified. Also available Low Endotoxin.

Applications

Approved:

IHC
IHC - Frozen
Immunoprecipitation
Flow Cytometry (1:50 - 1:100)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:	Mouse Concanavalin A activated spleen cells.
Specificity:	Recognizes the mouse Transferrin receptor protein 1 also known as CD71 or TfR1. CD71 is a 763 amino acid glycoprotein homodimer of ~95 kD subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies.
Usage:	Flow Cytometry: Use 10 ul of the suggested working dilution to label 1 x 10^6 cells in 100 ul. Method sheets are available on request.

Packaging

Presentation:	0.09% sodium azide.
Storage:	Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About CD71 / Transferrin Receptor

Uniprot: P02786 NCBI: NM_003234 NP_003225.2

Synonyms: TFRC Antibody, CD71 Antibody, TFR1 Antibody, TRFR Antibody, TFR Antibody, TR Antibody, CD71 antigen Antibody, p90 Antibody, T9 Antibody, Transferrin receptor Antibody, Transferrin receptor protein 1 Antibody

Description: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system By similarity.

(More About CD71 / Transferrin Receptor)

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