Target
Human CD7
Synonyms
CD7 | CD7 antigen | CD7 antigen (p41) | CD7 molecule | LEU-9 | T-cell leukemia antigen | T-cell antigen CD7 | T-cell surface antigen Leu-9 | TP41 | gp40 | p41 protein | Tp40
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence TEEQSQGWHRCSDAP, from the internal region of the protein sequence according to NP_006128.1.
Specificity
Human CD7. The immunizing peptide corresponds to a part of the extracellular domain.
Applications
- Western blot (0.5 - 2 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 37kD band observed in Human Thymus lysates (calculated MW of 25.4kD according to NP_006128.1). We do note that CD7 is a glycoprotein which makes a higher size band to be expected. The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee