Human CD65s

Mouse

Human (tested or 100% immunogen sequence identity)

IgM Monoclonal

VIM-2

FITC

Ammonium sulfate precipitation

Unmodified

THP1 (human acute monocytic leukaemia cells).

Selectively recognizes the sialylated form of human CD65, known as CD65s (VIM-2 antigen), a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukemic cells of myelomonocytic lineage. CD65s is aberrantly expressed on some acute myeloid leukemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34. Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation.

• Flow Cytometry (1:1 - 1:10)

The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, 0.09% Sodium Azide, 1% BSA

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

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