Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone VIM-2] (IgM) to Human CD65s
IHC - Frozen, Immunofluorescence, Flow Cytometry (applications tested for the base form of this product only)
Human (tested or 100% immunogen sequence identity)
IgM Monoclonal [VIM-2]
Ammonium sulfate precipitation
IHC - Frozen
Flow Cytometry (1:1 - 1:10)
(applications tested for the base form of this product only)
Specificity and Use
CD65s antibody was raised against tHP1 (human acute monocytic leukaemia cells).
Selectively recognizes the sialylated form of human CD65, known as CD65s (VIM-2 antigen), a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukemic cells of myelomonocytic lineage. CD65s is aberrantly expressed on some acute myeloid leukemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34. Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation.
The applications listed have been tested for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.
PBS, 0.09% sodium azide, 1% BSA
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. Protect from light.