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Anti-CD63 Antibody (clone AHN16.1/46-4-5, PE) LS-C35719

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Wt. Vol. Conc. Price
- 120 tst 1 mg/ml $495
Inquire for larger quantities

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Most Popular CD63 Antibodies

Anti-CD63 Antibody (clone AHN16.1/46-4-5, Biotin) LS-C35717
Mouse Monoclonal [clone AHN16.1/46-4-5] (IgG1) to Human CD63
Human
Flow Cytometry
Biotin Conjugated
Anti-CD63 Antibody LS-C204227
Goat Polyclonal (IgG) to Human CD63
Human, Monkey, Mouse, Rat, Dog
Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone AHN16.1/46-4-5] (IgG1) to Human CD63
Human
Flow Cytometry
PE Conjugated

Details

Human CD63
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [AHN16.1/46-4-5]
Phycoerythrin
Protein A purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

CD63 antibody was raised against human eosinophils.
Recognizes the CD63 molecule of about 50kD.
Suitable for use in Flow Cytometry. FC: Flow Cytometry: 1:50 (or use 1.6ul concentrated stock per 5x10e5 cells). 5x10e5 cultured HPB-MLT cells were washed and incubated 45mins. on ice with 80ul of LS-C35719 at a 1:50 dilution factor(20ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.70 log10fluorescent units when compared to a mouse IgG1/PE negativecontrol at a similar concentration. Binding was blocked when cellswere pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD63antibody (Cat. #LS-C35716). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, pH 7.2, 15% glycerol, 0.2% BSA, 0.04% sodium azide.
Do not freeze. Stable at 4º as an undiluted liquid. Dilute only prior to immediate use. Freezing PE conjugates will result in a substantial loss of enzymatic activity.
For research use only.

About CD63

P08962 NM_001780 NP_001771.1

CD63 Antibody, CD63 molecule Antibody, LAMP-3 Antibody, ME491 Antibody, Granulophysin Antibody, MLA1 Antibody, Tetraspanin-30 Antibody, Tspan-30 Antibody, CD63 antigen Antibody, OMA81H Antibody, TSPAN30 Antibody

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2.

Requested From: United States
Date Requested: 12/5/2016

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