Human CD59

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

FITC

Purified

Unmodified

Human erythrocytes.

Binds to a 17-25kD glycoprotein, which is distributed on human cells and has a glycosyl-phosphatidylinositol (GPI) linkage to the plasma membrane. Cell reactivity: Recognizes all leucocytes, platelets, fibroblasts, erythrocytes and most cell lines. It is negative on U937 and Daudi cell lines. It is reduced on affected cells from patients with PNH with a distribution reflecting the clonal nature of the disease.

• Immunofluorescence (1:25)
• Flow Cytometry

Studies of complement and homologous restriction of complement action of the properties of GPI linked proteins and in the characterization of cells from patients with PNH. Flow cytometry and indirect immunofluorescence: 1:25. Dilute with isotonic buffer. Use 50 ul of the appropriate dilution per 1x1E6 cells in 100 ul buffer. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P13987 NCBI: NM_000611 NP_000602.1