Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Flow Cytometry (1:25)
Specificity and Use
CD59 antibody was raised against human erythrocytes.
Binds to a 17-25kD glycoprotein which is distributed on human cells and has a glycosyl-phosphatidylinositol (GPI) linkage to the plasma membrane. Cell reactivity: Recognizes all leucocytes, platelets, fibroblasts, erythrocytes and most cell lines. It is negative on U937 and Daudi cell lines. It is reduced on affected cells from patients with PNH with a distribution reflecting the clonal nature of the disease.
Suitable for use in Flow Cytometry, Indirect Immunofluorescence, and ELISA. Flow Cytometry: 1:25. Indirect Immunofluorescence: 1:25. Dilute with isotonic buffer. Use 50 ul of the appropriate dilution per 1x1E6 cells in 100 ul buffer.
CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation.