Human CD58

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

TS2/9.1.4.3

FITC

Protein A purified

Unmodified

Human cytolytic T cells.

Human CD58 glycoprotein of about 60-70kD. Inhibits HLA-DR mediated T cell cytotoxicity

• Flow Cytometry

Flow Cytometry: 5x10e5 cultured Nalm-6 cells were washed after which they were incubated 45 min on ice with 80 ul of anti-CD58 antibody at 10 ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.46 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin

Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P19256 NCBI: NM_001779 NP_001770.1