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Anti-CD58 Antibody (clone TS2/9.1.4.3, FITC) LS-C189610

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular CD58 Antibodies

Anti-CD58 Antibody (clone TS2/9, Biotin) LS-C134511
Mouse Monoclonal [clone TS2/9] (IgG1) to Human CD58
Human
Flow Cytometry, Functional Assay
Biotin Conjugated
Flow Cytometry Image
Anti-CD58 Antibody (clone 2D11-B10) IHC-plus™ LS-B5386
Mouse Monoclonal [clone 2D11-B10] (IgG2a,k) to Human CD58
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone TS2/9.1.4.3] (IgG1) to Human CD58
Human
Flow Cytometry
FITC Conjugated

Details

Human CD58
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [TS2/9.1.4.3]
Fluorescein
Protein A purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

CD58 antibody was raised against human cytolytic T cells.
Human CD58 glycoprotein of about 60-70kD. Inhibits HLA-DR mediated T cell cytotoxicity
Flow Cytometry: 5x10e5 cultured Nalm-6 cells were washed after which they were incubated 45 min on ice with 80 ul of anti-CD58 antibody at 10 ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.46 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

50 mM sodium phosphate, pH 7.5, 100 mM potassium chloride, 150 mM sodium chloride, 0.5 mg/ml Gentamicin sulfate
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. Sensitive to light.
For research use only.

About CD58

P19256 NM_001779 NP_001770.1

CD58 Antibody, CD58 antigen Antibody, AG3 Antibody, LFA-3 Antibody, LFA3 Antibody, Surface glycoprotein LFA-3 Antibody, Surface glycoprotein lfa3 Antibody, CD58 molecule Antibody

Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.

Requested From: United States
Date Requested: 12/7/2016

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