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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD57 / HNK1 Antibody (clone 6‑13‑19‑1) IHC‑plus™ LS‑B8112
Note: This antibody replaces LS-C153033
HNK1 antibody LS-B8112 is an unconjugated mouse monoclonal antibody to human HNK1 (CD57). Validated for Ctox, IF and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B8112-500
500 µl
Unavailable

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Product Description

HNK1 antibody LS-B8112 is an unconjugated mouse monoclonal antibody to human HNK1 (CD57). Validated for Ctox, IF and IHC. Tested on 20 paraffin-embedded human tissues.
About CD57 / HNK1
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Q9P2W7 NM_018644 NP_061114.2

B3GAT1 Antibody, CD57 Antibody, CD57 antigen Antibody, GlcAT-P Antibody, GLCUATP Antibody, Glucuronosyltransferase P Antibody, HNK-1 Antibody, HNK1 Antibody, LEU7 Antibody, GLCATP Antibody, NK-1 Antibody, NK1 Antibody, LEU7 antigen Antibody, GlcUAT-P Antibody


Specifications

Target
Human CD57 / HNK1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgM Monoclonal [6-13-19-1]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:5)
  • Cytotoxic Assay (1:5)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD57 / HNK1 antibody was raised against jURKAT T-cell line
Specificity
Reacts with a 110 kD antigen present on the peripheral blood lymphocyte subset (primarily large granular lymphocytes) identified by the HNK-1 monoclonal antibody. It recognizes part of the Natural Killer cell population. CD57 is furthermore present on cells in neuroectodermal tissue. Positive control: peripheral bloodlymphocytes.
Usage
Dilution in phosphate buffered saline containing 1% BSA and 0.02% sodium azide is appropriate.
Presentation
Ascites (no preservative).
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CD57 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8112 dilution 1:50.
Anti-CD57 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8112 dilution 1:50.

Immunohistochemistry

Anti-CD57 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8112 dilution 1:50.
Anti-CD57 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8112 dilution 1:50.

Immunohistochemistry

Anti-CD57 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8112 dilution 1:50.
Anti-CD57 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8112 dilution 1:50.

Immunohistochemistry

Anti-CD57 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8112 dilution 1:50.
Anti-CD57 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8112 dilution 1:50.


Requested From: 
Date Requested: 6/18/2018

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