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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD52 Antibody (clone HI186, PE) LS‑C46505
CD52 antibody LS-C46505 is a PE-conjugated mouse monoclonal antibody to human CD52. Validated for Flow and IHC.
Catalog
Size
Price
LS-C46505-100
100 tst
$315
Description
CD52 antibody LS-C46505 is a PE-conjugated mouse monoclonal antibody to human CD52. Validated for Flow and IHC.
Target
Human CD52
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [HI186]
Conjugations
Phycoerythrin. Also available Unconjugated or conjugated with APC, FITC.
Modifications
Unmodified
Applications
  • IHC - Paraffin
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD52 antibody was raised against human tonsil
Specificity
The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa). CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.
Usage
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 15 mM Sodium Azide
Storage
Store in the dark at 2°C to 8°C. Do not freeze. Protect from light.
Restrictions
For research use only.
About CD52
P31358 NM_001803 NP_001794.2

Popular CD52 Products

Flow Cytometry Image
Species: Mouse
Applications: Immunoprecipitation, Flow Cytometry
Surface staining of CD52 in human peripheral blood with anti-CD52 (HI186) FITC.
Species: Human
Applications: IHC - Paraffin, Flow Cytometry
Antibody
Species: Mouse
Applications: Flow Cytometry
Flow cytometry of human peripheral blood lymphocytes with Rat anti-Human CD52: Low Endotoxin This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Monkey, Rhesus monkey
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Cytotoxic Assay
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Cytotoxic Assay

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Images

Flow Cytometry

Surface staining of CD52 in human peripheral blood with anti-CD52 (HI186) PE.
Surface staining of CD52 in human peripheral blood with anti-CD52 (HI186) PE.

Flow Cytometry

Surface staining of CD52 in human peripheral blood with anti-CD52 (HI186) PE.
Surface staining of CD52 in human peripheral blood with anti-CD52 (HI186) PE.

Flow Cytometry

Surface staining of CD52 in human peripheral blood with anti-CD52 (HI186) PE.
Surface staining of CD52 in human peripheral blood with anti-CD52 (HI186) PE.

Flow Cytometry

Surface staining of CD52 in human peripheral blood with anti-CD52 (HI186) PE.
Surface staining of CD52 in human peripheral blood with anti-CD52 (HI186) PE.

Requested From: United States
Date Requested: 12/11/2018
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