Human CD48

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

Unconjugated

Protein G purified

Unmodified

NS0-derived rhCD48.

Recognizes human CD48. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rmCD48 or rhOX40.

• Western blot
• Flow Cytometry
• ELISA

Western Blot, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect human CD48. Using a colorimetric detection system, the detection limit for rhCD48 is approximately 5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using blood-derived monocytes. Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human CD48. The detection limit for rhCD48 is approximately 10 ng/well.

Lyophilized from PBS, 5% Trehalose

Phosphate Buffered Saline (PBS)

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P09326 NCBI: NM_001778 NP_001769.2