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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD45 / LCA Antibody (clone YW62.3, FITC) LS‑C43230
Note: This antibody replaces LS-C57156, LS-C13534
Antibody
LCA antibody LS-C43230 is an FITC-conjugated rat monoclonal antibody to mouse LCA (CD45). Validated for Flow.
Catalog
Size
Price
LS-C43230-100
100 µg (0.1 mg/ml)
Unavailable

Popular CD45 / LCA Products

Anti-CD45 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2660 concentration 10 ug/ml.
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Anti-CD45 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-A9435 dilution 2.5-5 ug/ml.
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Anti-CD45 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8502 dilution 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry

Product Description

LCA antibody LS-C43230 is an FITC-conjugated rat monoclonal antibody to mouse LCA (CD45). Validated for Flow.
About CD45 / LCA
CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. P08575 NM_002838 NP_002829.3

PTPRC Antibody, B220 Antibody, CD45R Antibody, CD45 Antibody, gp180 Antibody, Leukocyte common antigen Antibody, L-CA Antibody, Leucocyte common antigen Antibody, LY5 Antibody, T200 Antibody, T200 glycoprotein Antibody, T200 leukocyte common antigen Antibody, CD45 antigen Antibody, LCA Antibody


Specifications

Target
Mouse CD45 / LCA
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [YW62.3]
Conjugations
Fluorescein
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Flow Cytometry (1:1)
Immunogen
CD45 / LCA antibody was raised against mouse spleen cells.
Specificity
Recognizes the murine CD45 cell surface antigen, a single pass type1 transmembrane glycoprotein also known as protein tyrosine phosphatase receptor type C (PTPRC) and originally termed Leucocyte Common Antigen (LCA). CD45 is a 180-220kD glycoprotein expressed by all leucocytes. CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains, this was subsequently expanded to include a third allele encoding Ly5.3. Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1. Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb;. A number of different isoforms isoforms of CD45 are expressed on murine leucocytes depending on the pattern of alternative splicing of 3 exons termed A, B and C encoding regions of ~50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product. Is reactive with all isoforms of murine CD45. N. B. Some reactivity with human tissue has been observed.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% sodium azide, 1% BSA.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/18/2018

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