Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone YTH24.5] (IgG2b) to Human CD45 / LCA
IHC, IHC - Paraffin, Flow Cytometry
Mouse Monoclonal [clone MRC OX-30] (IgG2a) to Rat CD45 / LCA
IHC, IHC - Frozen, Flow Cytometry
Rat CD45 / LCA
Rat (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [MRC OX-30]
Protein G purified
IHC - Frozen
Flow Cytometry (0.1 µg/10E6 cells)
Specificity and Use
CD45 / LCA antibody was raised against lymph node glycoproteins and cells.
Anti-rat CD45 monoclonal antibody recognizes a monomorphic determinant of the rat leukocyte common antigen. The antigen recognized is a heavily glycosylated membrane glycoprotein of molecular weight 170000 Da on thymocytes but molecular weight 170000-22.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 0.02% sodium azide, EIA grade BSA.
Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze/thaw cycles. Protect from light.
CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP.