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Anti-CD3E Antibody LS-C45012
Note: This antibody replaces LS-C58259Most Popular CD3E Antibodies
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Details
| Mouse CD3E | |
| Hamster | |
| Mouse (tested or 100% immunogen sequence identity) | |
| IgG Monoclonal | |
| Unconjugated. Also available conjugated with Biotin, Cy5, RPE. | |
| Protein G purified | |
| Unmodified |
Applications
- IHC
- IHC - Frozen
- Immunofluorescence
- Western blot
- Immunoprecipitation
- Flow Cytometry
- (applications tested for the base form of this product only)
Specificity and Use
| CD3E antibody was raised against h-2K b - specific mouse cytotoxic T lymphocyte clone BM10-37. | |
| Detects CD3 epsilon (CD3e), a 20kD transmembrane protein also known as CD3 or T3. CD3e is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell. CD3e is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions. | |
| Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. |
Packaging
| PBS, pH 7.2, 0.09% sodium azide. | |
| For research use only. |
About CD3E
NM_000733
NP_000724.1
CD3E Antibody, CD3e antigen Antibody, CD3-epsilon Antibody, T-cell receptor epsilon chain Antibody, TCRE Antibody, T-cell receptors epsilon chain Antibody, T3E Antibody, CD3 Epsilon Antibody
CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.
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Requested From:
Date Requested: 4/20/2018
Date Requested: 4/20/2018
