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Anti-CD3E Antibody (clone M2AB) LS-C140264

Ordering

Wt. Vol. Conc. Price
100 µg - - $265
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD3E Antibodies

Anti-CD3E Antibody (Azide-free) LS-C78117
Mouse Monoclonal (IgG1) to Human CD3E
Human
IHC, Immunoprecipitation, Flow Cytometry
Unconjugated, Azide-free
Anti-CD3E Antibody (Biotin) LS-C78118
Mouse Monoclonal (IgG1) to Human CD3E
Human
IHC, Flow Cytometry
Biotin Conjugated
Anti-CD3E Antibody (clone 3E10) LS-C174661
Mouse Monoclonal [clone 3E10] (IgG2b) to Human CD3E
Human, Monkey, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone M2AB] (IgG1) to Human CD3E
Human
Flow Cytometry
Unconjugated

Details

Human CD3E
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [M2AB]
Unconjugated
Protein A/G purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

CD3E antibody was raised against human thymocytes.
Human PGR / Progesterone Receptor
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2° to 8o C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use as above, however use only 5 ul per test rather than 10 l. Excites at 488nm and emits at 670nm. Store protected from light.

Packaging

PBS, 0.08% Sodium Azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About CD3E

NM_000733 NP_000724.1

CD3E Antibody, CD3e antigen Antibody, CD3-epsilon Antibody, T-cell receptor epsilon chain Antibody, TCRE Antibody, T-cell receptors epsilon chain Antibody, T3E Antibody, CD3 Epsilon Antibody

CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.

Requested From: United States
Date Requested: 12/4/2016

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