Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse (tested or 100% immunogen sequence identity)
Specificity and Use
Mouse CD3e (Mr 25kD). Recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.
Identification and enumeration of CD3+ cells by flow cytometry. Immunoprecipitation. In vitro depletion of CD3+ cells. In vitro activation of T cells (accessory cells must be provided). Immunohistochemistry. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, pH 7.4. No preservative added.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.