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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD3D Antibody LS‑C192500
CD3D antibody LS-C192500 is an unconjugated rabbit polyclonal antibody to CD3D from human, mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C192500-100
100 µl
Unavailable

Popular CD3D Products

Western blot of recombinant CD3D.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot
Immunofluorescent analysis of CD3d staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Monkey
Applications: ICC, Immunofluorescence, Western blot
Immunohistochemistry of CD3-delta antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Human, Monkey
Applications: ICC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA

Product Description

CD3D antibody LS-C192500 is an unconjugated rabbit polyclonal antibody to CD3D from human, mouse and rat. Validated for IHC and WB.
About CD3D
CD3D is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). P04234 NM_000732 NP_000723.1

CD3D Antibody, CD3 delta Antibody, CD3d antigen Antibody, T-cell receptor T3 delta chain Antibody, T3D Antibody, CD3 antigen, delta subunit Antibody, CD3-DELTA Antibody, OKT3, delta chain Antibody


Specifications

Target
Human CD3D
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD3D antibody was raised against synthetic peptide from human CD3D
Specificity
Human CD3D
Presentation
PBS, 0.1% sodium azide, 50% glycerol
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of CD3D pAb in extracts from Jurkat and HuT-78 cells.
Western blot of CD3D pAb in extracts from Jurkat and HuT-78 cells.

Western blot

Western blot of CD3D pAb in extracts from Jurkat and HuT-78 cells.
Western blot of CD3D pAb in extracts from Jurkat and HuT-78 cells.

Western blot

Western blot of CD3D pAb in extracts from Jurkat and HuT-78 cells.
Western blot of CD3D pAb in extracts from Jurkat and HuT-78 cells.

Western blot

Western blot of CD3D pAb in extracts from Jurkat and HuT-78 cells.
Western blot of CD3D pAb in extracts from Jurkat and HuT-78 cells.


Requested From: 
Date Requested: 6/24/2018

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