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| Catalog Number | Size | Price |
|---|---|---|
| LS-B8765-50 | 50 µg (1 mg/ml) | $605 |
PathPlus™ Monoclonal Rat anti‑Human CD3 Antibody (clone CD3‑12, IHC, WB) LS‑B8765
PathPlus™ Monoclonal Rat anti‑Human CD3 Antibody (clone CD3‑12, IHC, WB) LS‑B8765
Note: This antibody replaces LS-C121496
Antibody:
CD3 Rat anti-Human Monoclonal (CD3-12) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CD3 Rat anti-Human Monoclonal (CD3-12) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD3 is an integral membrane glycoprotein that associates with T-cell antigen receptor (TCR) and is required for TCR cell surface expression and signal transduction. It is a highly specific marker for normal T-lymphocytes, thymocytes, and T-cell malignancies. It is negative in B-cells.
References: Chetty and Gatter, 1994; Smith-Garvin et al. 2009
Host
Rat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
CD3-12
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Immunogen
Synthetic peptide sequence derived from cytoplasmic epitope of CD3.
Specificity
Recognizes a highly conserved epitope of the CD3 molecule expressed by T lymphocytes.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen (1:1000)
- Western blot (1:50 - 1:100)
- Flow Cytometry (1:50 - 1:100)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Western Blot. Flow Cytometry: 1:50-1:100; 10 ul labels 10^6 cells in 100ul. Immunohistochemistry (frozen): 1:1000.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-CD3 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD3 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
PathPlus™ CD3 Antibody (clone CD3-12) for IHC, WB/Western, Flow LS-B8765 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Frozen, Flow Cytometry, Functional Assay
Species:
Human
Applications:
Western blot, Flow Cytometry, Functional Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
Date Requested: 4/26/2024
