(tested or 100% immunogen sequence identity)
Also available Unconjugated
or conjugated with PE
Protein A/G purified
- Flow Cytometry
- (applications tested for the base form of this product only)
CD28 antibody was raised against hPB-ALL T-cell line.
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 0.08% sodium azide, 0.2% carrier protein, sterile-filtered
Store at 4°C. Do not freeze.
For research use only.