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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD28 Antibody (clone B‑23, FITC) LS‑C140327
CD28 antibody LS-C140327 is an FITC-conjugated mouse monoclonal antibody to human CD28. Validated for Flow.
Antibody
Catalog
Size
Price
LS-C140327-100
100 tst
$295

Popular CD28 Products

Anti-CD28 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunoprecipitation, Flow Cytometry, Functional Assay
Antibody
Species: Human
Applications:
Antibody
Species: Human
Applications: Flow Cytometry, ELISA, Functional Assay
Antibody
Species: Human
Applications: Flow Cytometry, ELISA
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry

Product Description

CD28 antibody LS-C140327 is an FITC-conjugated mouse monoclonal antibody to human CD28. Validated for Flow.
About CD28
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. P10747 NM_006139 NP_006130.1

CD28 Antibody, CD28 antigen Antibody, CD28 antigen (Tp44) Antibody, Tp44 Antibody, CD28 molecule Antibody


Specifications

Target
Human CD28
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [B-23]
Conjugations
Fluorescein. Also available Unconjugated or conjugated with PE.
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Immunogen
CD28 antibody was raised against hPB-ALL T-cell line.
Specificity
Human CD28
Usage
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.08% sodium azide, 0.2% carrier protein, sterile-filtered
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/25/2018

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