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Anti-CD276 / B7-H3 Antibody (clone MIH42) IHC-plus™ LS-B2614

Note: This antibody replaces LS-C58215, LS-C58217

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD276 / B7-H3 Antibodies

Anti-CD276 / B7-H3 Antibody (clone MIH42) IHC-plus™ LS-B2614
Mouse Monoclonal [clone MIH42] (IgG1) to Human CD276 / B7-H3
Human
IHC - Paraffin, IHC - Frozen, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-CD276 / B7-H3 Antibody (clone 6A1) LS-C169253
Mouse Monoclonal [clone 6A1] (IgG1) to Human CD276 / B7-H3
Human
IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CD276 / B7-H3 Antibody (clone SP206) LS-C210430
Rabbit Monoclonal [clone SP206] (IgG) to Human CD276 / B7-H3
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone MIH42] (IgG1) to Human CD276 / B7-H3
Human
IHC - Paraffin, IHC - Frozen, Flow Cytometry (applications tested for the base form of this product only)
Unconjugated

Details

Human CD276 / B7-H3
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MIH42]
Unconjugated. Also available conjugated with FITC, RPE.
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1:100)
  • Flow Cytometry (1:10 - 1:100)
  • (applications tested for the base form of this product only)

Specificity and Use

Detects CD276, a member of the B7 family of co-stimulatory molecules also known as B7-H3. CD276 is a type I transmembrane protein that induces the proliferation of CD4+ and CD8+ T cells, enhances the generation of cytotoxic T cells and selectively stimulates the production of interferon gamma. Expression of CD276 can be induced on dendritic cells and monocytes by inflammatory cytokines, and is also widely expressed in peripheral tissues including the heart, kidney, testis and colon. In humans, CD276 exists as two isoforms which result from gene duplication and differential splicing. CD276 is reported to have therapeutic potential for the regulation of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy.
Immunohistochemistry: LS-B2614 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2614 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Histology Positive Control Tissue: Acetone fixed Human Tonsil.

Packaging

PBS, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About CD276 / B7-H3

Q5ZPR3 NM_025240 NP_079516.1

CD276 Antibody, 4Ig-B7-H3 Antibody, B7 homolog 3 Antibody, B7-H3 Antibody, CD276 antigen Antibody, CD276 molecule Antibody, Costimulatory molecule Antibody, B7H3 Antibody, B7RP-2 Antibody

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.

Immunohistochemistry

Immunohistochemistry
Anti-B7-H3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2614 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/7/2016

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