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Anti-CD247 / CD3 Zeta Antibody (Cytoplasmic Domain, clone G3) LS-C5882

Ordering

Wt. Vol. Conc. Price
200 µg - - $665
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD247 / CD3 Zeta Antibodies

Anti-CD247 / CD3 Zeta Antibody (clone 4A12-F6) IHC-plus™ LS-B6021
Mouse Monoclonal [clone 4A12-F6] (IgG1,k) to Human CD247 / CD3 Zeta
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone G3] (IgG2a) to Human CD247 / CD3 Zeta
Human, Mouse
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated

Details

Human CD247 / CD3 Zeta
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [G3]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Frozen
  • Western blot
  • Immunoprecipitation (2 µg/ml)
  • Flow Cytometry (1:50)
  • ELISA (1 µg/ml)

Specificity and Use

CD247 / CD3 Zeta antibody was raised against 113 Amino acid synthetic peptide corresponding to the cytoplasmic domain of murine zeta.
Cytoplasmic Domain
The T cell antigen receptor (TCR) consists of a ligand-specific alpha/beta heterodimer non-covalently associated with five invariant chains including the CD3 gamma/delta/eta and zeta subunits, all of which are required for efficient surface expression. T cell activation through the TCR induces cellular differentiation and/or proliferation and the production of lymphokines and cytokines. Both the CD3 and TCR zeta subunits are proposed to be responsible for the intracellular signal transduction events. A novel monoclonal antibody, named 3H2012, has been developed that is specific for the TCR zeta chain subunit (CD247). The antibody is capable of precipitating both non-phosphorylated and tyrosine phosphorylated TCR zeta chain. Species cross-reactivity: Mouse and human.
Flow Cytometry: 1:50-1: ELISA: 1 ug/ml. Immunoprecipitation: 2 ug/ml. Western Blot. Immunohistochemistry (frozen).

Packaging

PBS, pH 7.2, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CD247 / CD3 Zeta

P20963 NM_000734 NP_000725.1

CD247 Antibody, CD3 Zeta Antibody, CD3H Antibody, CD3Z Antibody, CD247 antigen, zeta subunit Antibody, CD247 molecule Antibody, CD3zeta chain Antibody, T3Z Antibody, TCR zeta chain Antibody, TCRZ Antibody, T-cell receptor T3 zeta chain Antibody, CD247 antigen Antibody, CD3-ZETA Antibody, CD3Q Antibody

CD247 / CD3 Zeta is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Requested From: United States
Date Requested: 12/9/2016

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