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Anti-CD20 Antibody (clone 4A4) LS-C175329

Catalog Size Price
LS-C175329-100 100 µl (1 mg/ml) Unavailable

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Staining of BALB/c splenocytes with Alexa Fluor 647 anti-mouse CD19 (1D3) and 0.125 ug of PE Rat IgG2a Isotype control (left) or 0.125 ug PE anti-mouse CD20 (AISB12) (right). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Mouse Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed
Mouse Monoclonal [clone 4A4] (IgG1) to Human CD20
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human CD20
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [4A4]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (1:150)
  • Western blot (1:500)

Specificity and Use

CD20 antibody was raised against full length human recombinant protein of human MS4A1 (NP_068769) produced in HEK293T cell.
Human CD20

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About CD20

P11836 NM_021950 NP_690605.1

MS4A1 Antibody, B-lymphocyte antigen CD20 Antibody, B1 Antibody, Bp35 Antibody, CD20 Antibody, CD20 antigen Antibody, CD20 receptor Antibody, LEU-16 Antibody, CVID5 Antibody

CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members.

Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody.
IHC of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MS4A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MS4A1.

Requested From: 
Date Requested: 4/19/2018

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