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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD20 Antibody (clone 4A4) LS‑C175329
CD20 antibody LS-C175329 is an unconjugated mouse monoclonal antibody to human CD20. Validated for IHC and WB.
Catalog
Size
Price
LS-C175329-100
100 µl (1 mg/ml)
Unavailable

Popular CD20 Products

Antibody
Species: Human, Mouse, Rat
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry, ELISA
Anti-MS4A1 / CD20 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2605 dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Staining of BALB/c splenocytes with Alexa Fluor 647 anti-mouse CD19 (1D3) and 0.125 ug of PE Rat IgG2a Isotype control (left) or 0.125 ug PE anti-mouse CD20 (AISB12) (right). Cells in the lymphocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Flow Cytometry
Anti-CD20 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Mouse Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA

Product Description

CD20 antibody LS-C175329 is an unconjugated mouse monoclonal antibody to human CD20. Validated for IHC and WB.
About CD20
CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. P11836 NM_021950 NP_690605.1

MS4A1 Antibody, B-lymphocyte antigen CD20 Antibody, B1 Antibody, Bp35 Antibody, CD20 Antibody, CD20 antigen Antibody, CD20 receptor Antibody, LEU-16 Antibody, CVID5 Antibody


Specifications

Target
Human CD20
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [4A4]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:500)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD20 antibody was raised against full length human recombinant protein of human MS4A1 (NP_068769) produced in HEK293T cell.
Specificity
Human CD20
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody.
IHC of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MS4A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MS4A1.

Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody.
IHC of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MS4A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MS4A1.

Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody.
IHC of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MS4A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MS4A1.

Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody.
IHC of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MS4A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MS4A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MS4A1.


Requested From: 
Date Requested: 6/25/2018

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