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| Catalog Number | Size | Price |
|---|---|---|
| LS-C121508-200 | 200 µg | $797 |
Monoclonal Mouse anti‑Human CD19 Antibody (clone LE‑CD19, aa540‑557, IHC, WB) LS‑C121508
Monoclonal Mouse anti‑Human CD19 Antibody (clone LE‑CD19, aa540‑557, IHC, WB) LS‑C121508
Antibody:
CD19 Mouse anti-Human Monoclonal (aa540-557) (LE-CD19) Antibody
Application:
IHC, IHC-P, WB, IP, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CD19 Mouse anti-Human Monoclonal (aa540-557) (LE-CD19) Antibody
Application:
IHC, IHC-P, WB, IP, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CD19 antibody LS-C121508 is an unconjugated mouse monoclonal antibody to human CD19 (aa540-557). Validated for ELISA, Flow, IHC, IP and WB.
Target
Human CD19
Synonyms
CD19 | B-lymphocyte antigen CD19 | CD19 molecule | Differentiation antigen CD19 | T-cell surface antigen Leu-12 | B4 | CD19 antigen | CVID3
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
LE-CD19
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
CD19 peptide C-GPDPAWGGGGRMGTWSTR (C-Terminus) coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (94%); Elephant, Panda, Dog (89%); Pig (83%).
Epitope
aa540-557
Specificity
Recognizes the C-terminal cytoplasmic tail sequence (CGPDPAWGGGGRMGTWSTR) of human CD19, a 95kD cell surface glycoprotein, which is expressed by cells of the B cell lineage and follicular dendritic cells. CD19 is absent on plasma cells. Detects a band of ~95kD in Raji cell lysates under reducing conditions.
Applications
- IHC
- IHC - Paraffin (1:100 - 1:200)
- Western blot (1:100 - 1:200)
- Immunoprecipitation
- Flow Cytometry (1:100 - 1:200)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in ELISA, Western Blot, Immunohistochemistry (paraffin), Immunoprecipitation, and Flow Cytometry. Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ulImmunohistochemistry (paraffin): 1:100-1:200. Membrane permeabilization and antigen retrieval using heat treatment methods prior to staining of paraffin sections.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CD19
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Rat, Dog
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Mouse
Applications:
IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Species:
Mouse
Applications:
IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Mouse
Applications:
Immunoprecipitation, Flow Cytometry
Species:
Mouse
Applications:
Immunoprecipitation, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/8/2024
Date Requested: 5/8/2024
