Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1F4] (IgG1) to Human CD144 / CDH5 / VE Cadherin
IHC - Paraffin, Western blot
Mouse Monoclonal [clone BV9] (IgG2a) to Human CD144 / CDH5 / VE Cadherin
IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay (applications tested for the base form of this product only)
Human CD144 / CDH5 / VE Cadherin
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [BV9]
Also available conjugated with FITC.
IHC - Frozen
(applications tested for the base form of this product only)
Specificity and Use
Human CDH5 / VE Cadherin
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of VE-cadherin to be inactivated.
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% Triton X-100 (3 min, room temperature). Cells were incubated with a final concentration of 10 mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI. This image was taken for the unconjugated form of this product. Other forms have not been tested.