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Anti-CD144 / CDH5 / VE Cadherin Antibody (clone BV9) LS-C139964

Note: This antibody replaces LS-C122782

Ordering

Wt. Vol. Conc. Price
100 µg - 0.1 mg/ml $455
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD144 / CDH5 / VE Cadherin Antibodies

Anti-CD144 / CDH5 / VE Cadherin Antibody (aa1-258, FITC) LS-C40121
Rabbit Polyclonal (IgG) to Human CD144 / CDH5 / VE Cadherin
Human, Mouse, Rat, Bovine
Flow Cytometry
FITC Conjugated
Anti-CD144 / CDH5 / VE Cadherin Antibody (aa1-258) IHC-plus™ LS-B2138
Rabbit Polyclonal to Human CD144 / CDH5 / VE Cadherin
Human, Mouse, Rat, Bovine
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry, ELISA, Functional Assay
Unconjugated
Immunohistochemistry Image
Anti-CD144 / CDH5 / VE Cadherin Antibody (clone 16B1) IHC-plus™ LS-B3780
Mouse Monoclonal [clone 16B1] (IgG1) to Human CD144 / CDH5 / VE Cadherin
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone BV9] (IgG2a) to Human CD144 / CDH5 / VE Cadherin
Human
IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay (applications tested for the base form of this product only)
Unconjugated

Details

Human CD144 / CDH5 / VE Cadherin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [BV9]
Unconjugated. Also available conjugated with FITC.
Purified
Unmodified

Applications

  • IHC - Frozen
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
  • Functional Assay
  • (applications tested for the base form of this product only)

Specificity and Use

Human CDH5 / VE Cadherin
For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of VE-cadherin to be inactivated.

Packaging

PBS, 0.1% BSA
Store at 4°C, stable for one year.
For research use only.

About CD144 / CDH5 / VE Cadherin

P33151 NM_001795 NP_001786.2

CDH5 Antibody, 7B4 Antibody, 7B4 antigen Antibody, CD144 Antibody, Cadherin-5 Antibody, Endothelial-specific cadherin Antibody, Vascular endothelial cadherin Antibody, VE-cadherin Antibody, VEC Antibody, CD144 antigen Antibody, VE Cadherin Antibody

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.

Immunofluorescence

Immunofluorescence
Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% Triton X-100 (3 min, room temperature). Cells were incubated with a final concentration of 10 mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/2/2016

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