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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD144 / CDH5 / VE Cadherin Antibody (clone 16B1) IHC‑plus™ LS‑B3780 Best Seller
Note: This antibody replaces LS-C106636
VE Cadherin antibody LS-B3780 is an unconjugated mouse monoclonal antibody to human VE Cadherin (CD144 / CDH5). Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Catalog
Size
Price
LS-B3780-100
100 µg (0.5 mg/ml)
Unavailable

Popular CD144 / CDH5 / VE Cadherin Products

Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% Triton X-100 (3 min, room temperature). Cells were incubated with a final concentration of 10 mg/ml BV9. Secondary detection was performed with anti-mouse Alexa-Fluor 647 and counterstained using DAPI.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay
IHC of p-VE-Cadherin (Y731) pAb in paraffin-embedded human brain tissue.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
CD144 / CDH5 / VE Cadherin antibody. IHC(P): Rat Brain Tissue.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-CDH5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

VE Cadherin antibody LS-B3780 is an unconjugated mouse monoclonal antibody to human VE Cadherin (CD144 / CDH5). Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
About CD144 / CDH5 / VE Cadherin
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. P33151 NM_001795 NP_001786.2

CDH5 Antibody, 7B4 Antibody, 7B4 antigen Antibody, CD144 Antibody, Cadherin-5 Antibody, Endothelial-specific cadherin Antibody, Vascular endothelial cadherin Antibody, VE-cadherin Antibody, VEC Antibody, CD144 antigen Antibody, VE Cadherin Antibody


Specifications

Target
Human CD144 / CDH5 / VE Cadherin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [16B1]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (20 µg/ml)
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human CDH5
Usage
Immunohistochemistry: LS-B3780 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3780 was determined to be 20 ug/ml.Tested in Western blot using Human Umbilical Vein Endothelial Cell (HUVEC) lysates.
Presentation
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Storage
Store at 2-8°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
A Hereditary Enteropathy Caused by Mutations in the SLCO2A1 Gene, Encoding a Prostaglandin Transporter. Umeno J, Hisamatsu T, Esaki M, Hirano A, Kubokura N, Asano K, Kochi S, Yanai S, Fuyuno Y, Shimamura K, Hosoe N, Ogata H, Watanabe T, Aoyagi K, Ooi H, Watanabe K, Yasukawa S, Hirai F, Matsui T, Iida M, Yao T, Hibi T, Kosaki K, Kanai T, Kitazono T, Matsumoto T. PLoS genetics. 2015 11:e1005581. (IHC, IF; Human) [Full Text Article] [PubMed:26539716] [PMC:PMC4634957]

Customer Reviews (0)


Images


Immunohistochemistry

Anti-VE Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.
Anti-VE Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.

Immunohistochemistry

Anti-VE Cadherin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.
Anti-VE Cadherin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.

Western blot

EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were lysed, and lysates were loaded at 1x105 cells/lane, probed with 2 ug/mL purified 16B1 and revealed with HRP anti-rat IgG.
EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were lysed, and lysates were loaded at 1x105 cells/lane, probed with 2 ug/mL purified 16B1 and revealed with HRP anti-rat IgG.

Immunohistochemistry

Anti-VE Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.
Anti-VE Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.

Immunohistochemistry

Anti-VE Cadherin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.
Anti-VE Cadherin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.

Western blot

EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were lysed, and lysates were loaded at 1x105 cells/lane, probed with 2 ug/mL purified 16B1 and revealed with HRP anti-rat IgG.
EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were lysed, and lysates were loaded at 1x105 cells/lane, probed with 2 ug/mL purified 16B1 and revealed with HRP anti-rat IgG.

Immunohistochemistry

Anti-VE Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.
Anti-VE Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.

Immunohistochemistry

Anti-VE Cadherin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.
Anti-VE Cadherin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.

Western blot

EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were lysed, and lysates were loaded at 1x105 cells/lane, probed with 2 ug/mL purified 16B1 and revealed with HRP anti-rat IgG.
EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were lysed, and lysates were loaded at 1x105 cells/lane, probed with 2 ug/mL purified 16B1 and revealed with HRP anti-rat IgG.

Immunohistochemistry

Anti-VE Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.
Anti-VE Cadherin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.

Immunohistochemistry

Anti-VE Cadherin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.
Anti-VE Cadherin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3780 concentration 20 ug/ml.

Western blot

EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were lysed, and lysates were loaded at 1x105 cells/lane, probed with 2 ug/mL purified 16B1 and revealed with HRP anti-rat IgG.
EDTA-passaged (left) and Trypsin-passaged (right) Human Umbilical Vein Endothelial Cells (HUVEC) were lysed, and lysates were loaded at 1x105 cells/lane, probed with 2 ug/mL purified 16B1 and revealed with HRP anti-rat IgG.


Requested From: 
Date Requested: 6/20/2018

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