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Anti-CD14 Antibody (aa292-322) LS-C100022

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

Most Popular CD14 Antibodies

Anti-CD14 Antibody (PE) LS-C4609
Mouse Monoclonal (IgG2a) to Human CD14
Human, Monkey, Bovine, Cat, Dog, Pig, Sheep
Flow Cytometry
PE Conjugated
Anti-CD14 Antibody (clone TÜK4, FITC) LS-C43762
Mouse Monoclonal [clone TÜK4] (IgG2a) to Human CD14
Human, Bovine, Cat, Dog, Goat, Mink, Pig, Rabbit, Sheep, Cynomolgus monkey, Llama
Flow Cytometry
FITC Conjugated
Anti-CD14 Antibody (aa57-64, clone MEM-18, FITC) LS-C46346
Mouse Monoclonal [clone MEM-18] (IgG1) to Human CD14
Human, Primate
Flow Cytometry
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CD14
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human CD14
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

  • IHC - Paraffin

Specificity and Use

LS-E8189 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C100022. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa292-322
This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-322 amino acids from the C-terminal region of human CD14.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About CD14

P08571 NM_000591 NP_000582.1

CD14 Antibody, CD14 molecule Antibody, CD14 antigen Antibody

In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot
CD14 Antibody western blot of A549 cell line lysates (35 ug/lane). The CD14 antibody detected the CD14 protein (arrow).

Western blot

Western blot
Western blot of CD14(arrow) using rabbit polyclonal CD14 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).

Flow Cytometry

Flow Cytometry
CD14 Antibody flow cytometry of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: 
Date Requested: 3/30/2017

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