Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (5 - 10 µg/ml)
Western blot (2 µg/ml)
Specificity and Use
CD11b + c antibody was raised against a synthetic peptide made to an internal region (aa500-600) of the mouse CD11 (b/c) protein.
Recognizes CD11b/c. Using macrophage lysates, a doublet is seen ~95 and 103kD. A weaker doublet is also seen ~130 and 160kD. Species cross-reactivity: human and mouse. Cellular Localization: monocytes and granulocytes.
Suitable for use in Western Blot, Immunohistochemistry. Western Blot: 2 ug/ml. Immunohistochemistry (regular/paraffin): 5-10 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Tris-glycine, 150 mM sodium chloride, 0.05% sodium azide. Labeled, HRP
Store at 2-8°C. Do Not Freeze! Aliquots are stable for at least 3 months. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates