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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD105 Antibody (clone 3A9) LS‑C169371
Note: This antibody replaces LS-C171606
CD105 antibody LS-C169371 is an unconjugated mouse monoclonal antibody to human CD105. Validated for ELISA, Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C169371-100
100 µl
Unavailable

Popular CD105 Products

Antibody
Species: Human, Pig
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Anti-ENG / CD105 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2165 dilution 1:100.
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Formalin-fixed, paraffin-embedded human tonsil stained with peroxidase-conjugate and AEC chromogen. Note staining of endothelial cells.
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Anti-CD105 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9998 concentration 10 ug/ml.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

CD105 antibody LS-C169371 is an unconjugated mouse monoclonal antibody to human CD105. Validated for ELISA, Flow, IF, IHC and WB.
About CD105
CD105 is a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. P17813 NM_000118 NP_000109.1

ENG Antibody, CD105 Antibody, CD105 antigen Antibody, END Antibody, HHT1 Antibody, ORW Antibody, ORW1 Antibody, Osler-Rendu-Weber syndrome 1 Antibody, Endoglin Antibody


Specifications

Target
Human CD105
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [3A9]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD105 antibody was raised against purified recombinant fragment of human CD105 expressed in E. Coli.\
Specificity
Human
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CD105 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using CD105 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using CD105 monoclonal antibody against HEK293 (1) and CD105(AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CD105 monoclonal antibody against HEK293 (1) and CD105(AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CD105 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using CD105 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using CD105 monoclonal antibody against HEK293 (1) and CD105(AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CD105 monoclonal antibody against HEK293 (1) and CD105(AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CD105 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using CD105 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using CD105 monoclonal antibody against HEK293 (1) and CD105(AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CD105 monoclonal antibody against HEK293 (1) and CD105(AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence of HepG2 cells using CD105 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence of HepG2 cells using CD105 mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot using CD105 monoclonal antibody against HEK293 (1) and CD105(AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using CD105 monoclonal antibody against HEK293 (1) and CD105(AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);


Requested From: 
Date Requested: 6/19/2018

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