(tested or 100% immunogen sequence identity)
Dog, Hamster, Horse (at least 90% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500)
- Flow Cytometry (1:12)
- ELISA (1:30000)
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CCR4 antibody was raised against 17SNYYLYESIPKPCTKEGIKAFGE39 corresponding to human CCR4 extracellular domain. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Gibbon, Panda (96%); Hamster, Dog, Horse (91%); Elephant, Rabbit (87%); Mouse (83%).
Peptide sequence is <50% identical to other chemokine receptors in this region.
Immunohistochemistry: LS-B4033 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4033 was determined to be 5 ug/ml.
10 mM KHPO4, 140 mM NaCl with 1 mg/ml BSA and 0.1% sodium azide
Short term: -20°C; Long term: -70°C; Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.