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Anti-CCNB1 / Cyclin B1 Antibody (phospho-Ser126) LS-C18812

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCNB1 / Cyclin B1 Antibodies

Anti-CCNB1 / Cyclin B1 Antibody (Azide-free) LS-C22501
Mouse Monoclonal (IgG2a) to Human CCNB1 / Cyclin B1
Human, Mouse, Rat, Hamster
IHC - Paraffin, Immunofluorescence, Immunoprecipitation
Unconjugated, Azide-free
Anti-CCNB1 / Cyclin B1 Antibody (clone V152) IHC-plus™ LS-B8608
Mouse Monoclonal [clone V152] (IgG1) to Hamster CCNB1 / Cyclin B1
Hamster, Human, Mouse, Rat
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-CCNB1 / Cyclin B1 Antibody (aa145-149) LS-C205411
Rabbit Polyclonal to Human CCNB1 / Cyclin B1
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CCNB1 / Cyclin B1
Human, Mouse, Rat, Dog, Hamster
Western blot, ELISA
Unconjugated

Details

Human CCNB1 / Cyclin B1
Rabbit
Human, Mouse, Rat, Dog, Hamster (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:100 - 1:1000)
  • ELISA (1:50000)

Specificity and Use

CCNB1 / Cyclin B1 antibody was raised against synthetic peptide from human CCNB1 / Cyclin B1.
pSer126
Amino acids 120-131 of Human Cyclin B1 protein.
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band - 48 kD in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About CCNB1 / Cyclin B1

P14635 NM_031966 NP_114172.1

CCNB1 Antibody, CCNB Antibody, Cyclin B1 Antibody, G2/mitotic-specific cyclin-B1 Antibody, G2/mitotic-specific cyclin B1 Antibody

CCNB1 / Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Western blot

Western blot
Anti-Cyclin B1 pS126 Antibody - Western Blot. Western blot of Affinity Purified anti-Cyclin B1 pS126 antibody shows detection of a band ~48 kD corresponding to phosphorylated human Cyclin B1 (arrowheads) in various whole cell lysates. Lysates tested were lane 1 - HeLa (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast). Each lane contains approximately 50 ug of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel. Transfer occurred overnight at 4C at 15 mAmps. The membrane was blocked with 5% non-fat dry milk in TTBS for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature. After washes with TTBS a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX (LS-C60865) was added for 1 h at room temperature. After additional washes the membrane was incubated with ECL mix 1:1 for ~3 min. Excess detection solution was drained off and the membrane was exposed to Kodak film Xomat blue XB-1 for about 20 sec. Other detection systems will yield similar results. Personal Communication, Luca Cote, Temple U.
 

Requested From: United States
Date Requested: 12/7/2016

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