Human CCL7 / MCP3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein A purified

Unmodified

E.coli derived recombinant Human MCP-3.

Human MCP-3

• Western blot (0.5 - 1 µg/ml)
• ELISA (2 - 4 µg/ml)

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human MCP-3 (100 ng/ml), a concentration of 1-3 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect recombinant human MCP-3 when used with biotinylated antigen affinity purified anti-human MCP-3 (LS-C104664) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.5-1 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 2-4 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.

Lyophilized from PBS

Reconstitute with sterile water

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P80098 NCBI: NM_006273 NP_006264.2