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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCL7 / MCP3 Antibody (Biotin) LS‑C104662
Note: This antibody replaces LS-C54189, LS-C42365, LS-C37322
MCP3 antibody LS-C104662 is a biotin-conjugated goat polyclonal antibody to human MCP3 (CCL7). Validated for ELISA and WB.
Catalog
Size
Price
LS-C104662-25
25 µg
Unavailable
LS-C104662-50
50 µg
Unavailable

Popular CCL7 / MCP3 Products

Anti-CCL7 / MCP3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B930 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western Blot (reducing) of CCL7 / MCP-3 antibody LS-C104607.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Western blot, ELISA, Neutralization
Western Blot (reducing) of CCL7 / MCP-3 antibody LS-C104607.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Western blot, ELISA, Neutralization
Western blot analysis of CCL7 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Species: Human, Monkey, Mouse, Rat
Applications: Western blot
Western blot of CCL7 antibody at 1:500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: sp2/0 whole cell lysate. Lane 4: PC12 whole cell lysate.
Species: Human, Mouse, Rat
Applications: Western blot

Product Description

MCP3 antibody LS-C104662 is a biotin-conjugated goat polyclonal antibody to human MCP3 (CCL7). Validated for ELISA and WB.
About CCL7 / MCP3
CCL7 / MCP3 is monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. P80098 NM_006273 NP_006264.2

CCL7 Antibody, C-C motif chemokine 7 Antibody, Chemokine (C-C motif) ligand 7 Antibody, FIC Antibody, MARC Antibody, MCP3 Antibody, Monocyte chemotactic protein 3 Antibody, SCYA7 Antibody, Small-inducible cytokine A7 Antibody, MCP-3 Antibody, SCYA6 Antibody, NC28 Antibody


Specifications

Target
Human CCL7 / MCP3
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CCL7 / MCP3 antibody was raised against e.coli derived recombinant Human MCP-3.
Specificity
Human MCP-3
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hMCP-3 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-3. To detect hMCP-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MCP-3 (LS-C104663) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-3. Western Blot: To detect by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot (non-reducing) of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of CCL7 / MCP-3 antibody LS-C104663.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of CCL7 / MCP-3 antibody LS-C104663. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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