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Anti-CCL3 / MIP-1-Alpha Antibody LS-C154130

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCL3 / MIP-1-Alpha Antibodies

Anti-CCL3 / MIP-1-Alpha Antibody LS-C25331
Mouse Monoclonal (IgG1) to Human CCL3 / MIP-1-Alpha
Human
ELISA
Unconjugated
Anti-CCL3 / MIP-1-Alpha Antibody LS-C50375
Rabbit Polyclonal to Human CCL3 / MIP-1-Alpha
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-CCL3 / MIP-1-Alpha Antibody LS-C104637
Rabbit Polyclonal to Human CCL3 / MIP-1-Alpha
Human
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse CCL3 / MIP-1-Alpha
Mouse
Western blot, ELISA
Unconjugated

Details

Mouse CCL3 / MIP-1-Alpha
Rabbit
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • Western blot (1:1000)
  • ELISA

Specificity and Use

CCL3 / MIP-1-Alpha antibody was raised against this IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse MIP-1a protein produced in E. coli.
This antibody is specific for mouse MIP-1a protein. Cross-reactivity with MIP-1a from other sources has not been determined.
This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7-8 kD in size corresponding to the mature 92 amino acid mouse MIP-1a/Ccl3 protein by western blotting in appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CCL3 / MIP-1-Alpha

NM_002983 NP_002974.1

CCL3 Antibody, C-C motif chemokine 3 Antibody, G0S19-1 Antibody, Mip1alpha Antibody, SCYA3 Antibody, SIS-beta Antibody, Chemokine (C-C motif) ligand 3 Antibody, LD78ALPHA Antibody, MIP-1-alpha Antibody, MIP1A Antibody, PAT 464.1 Antibody, Small-inducible cytokine A3 Antibody

Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

Western blot

Western blot
Anti-Mouse MIP-1a Antibody - Western Blot. Anti-mouse MIP-1a in western blot shows detection of recombinant mouse MIP-1a/Ccl3 raised in E. coli. Recombinant truncated (0.1 ug, 7.8 kD) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
 

Requested From: United States
Date Requested: 12/8/2016

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