Target
Human CCL3 / MIP-1-Alpha
Synonyms
CCL3 | C-C motif chemokine 3 | G0S19-1 | Mip1alpha | SCYA3 | SIS-beta | Chemokine (C-C motif) ligand 3 | LD78ALPHA | MIP-1-alpha | MIP1A | PAT 464.1 | Small-inducible cytokine A3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
E.coli derived recombinant Human MIP-1alpha.
Specificity
Human MIP-1alpha
Applications
- Western blot (2 - 4 µg/ml)
- ELISA (2 - 4 µg/ml)
Usage
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human MIP-1alpha (100 ng/ml), a concentration of 5-10 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 2000 pg/ml of recombinant human MIP-1alpha when used with biotinylated antigen affinity purified anti-human MIP-1alpha (LS-C104668) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect Human MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 2-4 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-1alpha is 1-2 ng/lane, under non-reducing conditions.
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee