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Anti-CAV3 / Caveolin 3 Antibody (N-Terminus) IHC-plus™ LS-B8308

Note: This antibody replaces LS-C112836

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
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CAV3 / Caveolin 3 Antibody LS-B8308 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-Caveolin 3 antibody IHC of human skeletal muscle.
Western blot
CAV3 antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein/ml in RIPA buffer).

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Goat Polyclonal to Human CAV3 / Caveolin 3
Human, Monkey, Bovine, Dog, Horse
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CAV3 / Caveolin 3
Goat
Human, Monkey, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Bat, Pig, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (3.75 µg/ml), Western blot (0.3 - 1 µg/ml), ELISA (1:4000)

Specificity and Use

CAV3 / Caveolin 3 antibody was raised against synthetic peptide EHTDLEAQIVKDIH-C from the N-terminus of human CAV3 / Caveolin 3 (NP_203123.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Dog, Bovine, Horse (100%); Bat, Rabbit, Pig (93%); Mouse, Rat (86%).
LS-E27043 - Lyophilized - 100 µg - $145.00
N-Terminus
Human CAV3 / Caveolin 3. Reported variants represent identical protein (NP_001225.1; NP_203123.1).
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx. 23kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 17.3kDa according to NP_203123.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About CAV3 / Caveolin 3

P56539 NM_001234 NP_001225.1

CAV3 Antibody, Caveolin-3 Antibody, Caveolin3 Antibody, Caveolin 3 Antibody, LGMD1C Antibody, VIP-21 Antibody, VIP21 Antibody, LQT9 Antibody, M-caveolin Antibody

Caveolin proteins are components of caveolae plasma membranes found in most cell types. Caveolin-3 is the muscle-specific form of the caveolin protein family whose members are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules within the caveolae membranes. Mutations in caveolin-3 have been isolated which interfere with caveolin-3 oligomerization and disrupt caveolae formation resulting in limb-girdle muscular dystrophy.

Immunohistochemistry

Immunohistochemistry
Anti-Caveolin 3 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8308 dilution 3.75 ug/ml.

Western blot

Western blot
CAV3 antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 4/25/2015

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