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Anti-Carboxylesterase 1 / CES1 Antibody (aa533-544) IHC-plus™ LS-B2401

Note: This antibody replaces LS-C61719

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

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Most Popular Carboxylesterase 1 / CES1 Antibodies

Anti-Carboxylesterase 1 / CES1 Antibody (aa533-544) IHC-plus™ LS-B2401
Goat Polyclonal to Human Carboxylesterase 1 / CES1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

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Goat Polyclonal to Human Carboxylesterase 1 / CES1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human Carboxylesterase 1 / CES1
Goat
Human (tested or 100% immunogen sequence identity)
Monkey, Horse (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • ELISA (1:8000)

Specificity and Use

Carboxylesterase 1 / CES1 antibody was raised against synthetic peptide C-NTQAAQKLKDKE from an internal region (near C-terminus) of human CES1 (NP_001020366.1; NP_001020365.1; NP_001257.4). Percent identity by BLAST analysis: Human, Orangutan, Monkey (100%); Gorilla, Gibbon, Marmoset, Horse (92%); Mouse, Rat, Hamster, Panda, Bovine, Cat (83%).
LS-E26652 - Lyophilized - 100 µg - $145.00
aa533-544
Human CES1. This antibody is expected to recognise all three reported isoforms (NP_001020366.1; NP_001020365.1; NP_001257.4).
Immunohistochemistry: LS-B2401 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2401 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About Carboxylesterase 1 / CES1

P23141 L07764 AAA16036.1

CES1 Antibody, ACAT Antibody, Brain carboxylesterase hBr1 Antibody, Carboxylesterase 1 Antibody, Carboxylesterase 2 (liver) Antibody, CEH Antibody, CES1A1 Antibody, CES1A2 Antibody, Cocaine carboxylesterase Antibody, Egasyn Antibody, HMSE Antibody, Liver carboxylesterase 1 Antibody, HCE-1 Antibody, PCE-1 Antibody, Retinyl ester hydrolase Antibody, TGH Antibody, Triacylglycerol hydrolase Antibody, REH Antibody, SES1 Antibody, CE-1 Antibody, Cholesteryl ester hydrolase Antibody, HMSE1 Antibody, Serine esterase 1 Antibody

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.

Immunohistochemistry

Immunohistochemistry
Anti-CES1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2401 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.03 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/7/2016

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