Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1) to Bovine CAPN1 / Calpain 1
IHC, ICC, Western blot, Immunoprecipitation, ELISA
Bovine CAPN1 / Calpain 1
Bovine (tested or 100% immunogen sequence identity)
Western blot (1:500 - 1:1000)
Specificity and Use
CAPN1 / Calpain 1 antibody was raised against bovine myocardial mu-calpain.
Reacts with the small subunit of human erythrocyte u-calpain or bovine myocardial m-calpain. Does not react with calpain large subunits.
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation. Western Blot: 1:500-1:1000. Recognizes the 30kD small subunits of either calpain isozyme. ELISA: 1:50000. Immunohistochemistry: 1:100 on 4% paraformaldehyde or 1% paraformaldehyde/4% glutaraldehyde fixed tissue. Immunocytochemistry: 1:100. Immunoprecipitation: (small unit, in the presence of calcium large & small complex).
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.