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Catalog Number Size Price
LS-C21156-200 200 µg $919 
Antibody

Polyclonal Rabbit anti‑Human CALR / Calreticulin Antibody (C‑Terminus, IHC, WB) LS‑C21156

Polyclonal Rabbit anti‑Human CALR / Calreticulin Antibody (C‑Terminus, IHC, WB) LS‑C21156

Antibody:
CALR / Calreticulin Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Xenopus
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$919
LS-C21156-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CALR / Calreticulin Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat, Bovine, Pig, Xenopus
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Calreticulin antibody LS-C21156 is an unconjugated rabbit polyclonal antibody to Calreticulin (CALR) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human CALR / Calreticulin
Synonyms
CALR | Calregulin | CC1qR | CRP55 | CRT | CRTC | ERp60 | Grp60 | RO | SSA | Calreticulin | HACBP
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Pig, Xenopus (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Synthetic peptide (QAKDEL) corresponding to the C-Terminus (aa 412-417) of human calreticulin, KLH conjugated.
Epitope
C-Terminus
Specificity
Specific for calreticulin. Does not cross-react with other endoplasmic reticulum proteins. Species cross-reactivity: Human, mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.
Applications
  • IHC
  • Western blot (0.5 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for Western Blot and Immunohistochemistry. Western Blot: 0.5-2 ug/ml detects calreticulin in RIPA lysates from murine 3T3/NIH fibroblasts and human foreskin fibroblast (HFF). 20 ug 3T3/NIH and 10 ug HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-human Calreticulin (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Detects calreticulin in tissue sections and cells. Included Positive Antigen Control: 3T3 lysate. Use 20 ug per lane for minigels.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CALR / Calreticulin
P27797 NM_004343 NP_004334.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/19/2024