Human CALR / Calreticulin

Rabbit

Human, Mouse, Rat, Bovine, Pig, Xenopus (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Synthetic peptide (QAKDEL) corresponding to the C-Terminus (aa 412-417) of human calreticulin, KLH conjugated.

C-Terminus

Specific for calreticulin. Does not cross-react with other endoplasmic reticulum proteins. Species cross-reactivity: Human, mouse, rat, Xenopus and weakly with bovine and porcine. No reaction with chicken.

• IHC
• Western blot (0.5 - 2 µg/ml)

Suitable for Western Blot and Immunohistochemistry. Western Blot: 0.5-2 ug/ml detects calreticulin in RIPA lysates from murine 3T3/NIH fibroblasts and human foreskin fibroblast (HFF). 20 ug 3T3/NIH and 10 ug HFF cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-human Calreticulin (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunohistochemistry: Detects calreticulin in tissue sections and cells. Included Positive Antigen Control: 3T3 lysate. Use 20 ug per lane for minigels.

0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P27797 NCBI: NM_004343 NP_004334.1