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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CALB1 / Calbindin Antibody (clone 1F6) LS‑C196804
Calbindin antibody LS-C196804 is an unconjugated mouse monoclonal antibody to human Calbindin (CALB1). Validated for ELISA and WB.
Catalog
Size
Price
LS-C196804-100
100 µg
Unavailable

Popular CALB1 / Calbindin Products

Antibody
Species: Chicken
Applications: IHC
Anti-CALB1 / Calbindin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5186 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-CALB1 / Calbindin antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6725 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Western blot of recombinant CALB1 / Calbindin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Rat
Applications: IHC, Western blot
Western blot of recombinant CALB1 / Calbindin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Western blot

Product Description

Calbindin antibody LS-C196804 is an unconjugated mouse monoclonal antibody to human Calbindin (CALB1). Validated for ELISA and WB.
About CALB1 / Calbindin
CALB1 / Calbindin is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. P05937 NM_004929 NP_004920.1

CALB1 Antibody, Calbindin D28 Antibody, Calbindin Antibody, Calbindin 1, 28kDa Antibody, CAB27 Antibody, CALB Antibody, RTVL-H protein Antibody, Calbindin 1, (28kD) Antibody, D-28K Antibody


Specifications

Target
Human CALB1 / Calbindin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [1F6]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
CALB1 (NP_004920.1, aa1-261 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human Calbindin
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of CALB1 expression in transfected 293T cell line by CALB1 monoclonal antibody (M01), clone 1F6.Lane 1: CALB1 transfected lysate (Predicted MW: 30 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CALB1 expression in transfected 293T cell line by CALB1 monoclonal antibody (M01), clone 1F6.Lane 1: CALB1 transfected lysate (Predicted MW: 30 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CALB1 is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CALB1 is 0.3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CALB1 expression in transfected 293T cell line by CALB1 monoclonal antibody (M01), clone 1F6.Lane 1: CALB1 transfected lysate (Predicted MW: 30 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CALB1 expression in transfected 293T cell line by CALB1 monoclonal antibody (M01), clone 1F6.Lane 1: CALB1 transfected lysate (Predicted MW: 30 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CALB1 is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CALB1 is 0.3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CALB1 expression in transfected 293T cell line by CALB1 monoclonal antibody (M01), clone 1F6.Lane 1: CALB1 transfected lysate (Predicted MW: 30 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CALB1 expression in transfected 293T cell line by CALB1 monoclonal antibody (M01), clone 1F6.Lane 1: CALB1 transfected lysate (Predicted MW: 30 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CALB1 is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CALB1 is 0.3 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CALB1 expression in transfected 293T cell line by CALB1 monoclonal antibody (M01), clone 1F6.Lane 1: CALB1 transfected lysate (Predicted MW: 30 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CALB1 expression in transfected 293T cell line by CALB1 monoclonal antibody (M01), clone 1F6.Lane 1: CALB1 transfected lysate (Predicted MW: 30 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CALB1 is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CALB1 is 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/18/2018

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