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Anti-c-Met Antibody (phospho-Tyr1349/Tyr1356) LS-C60102


Wt. Vol. Conc. Price
100 µg - 1.47 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular c-Met Antibodies

Anti-c-Met Antibody (Cytoplasmic Domain) IHC-plus™ LS-B2812
Mouse Monoclonal (IgG2a) to Human c-Met
IHC - Paraffin, IHC - Frozen
Immunohistochemistry Image
Anti-c-Met Antibody (phospho-Tyr1234/Tyr1235) LS-C150281
Rabbit Polyclonal (IgG) to Human c-Met
Human, Mouse
IHC, Western blot, Flow Cytometry
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human c-Met
Human, Mouse, Rat
Western blot, ELISA


Human c-Met
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Western blot (1:500 - 1:2000)
  • ELISA (1:450000)

  • IHC - Paraffin

Specificity and Use

c-Met antibody was raised against synthetic peptide corresponding to residues surrounding Y1349 and Y1356 of human c-Met protein.
A BLAST analysis was used to suggest cross-reactivity with c-Met from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with c-Met from other sources has not been determined
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 150 kD in size corresponding to phosphorylated c-Met protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human c-Met pY1349pY1356 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About c-Met

P08581 NM_000245 NP_000236.2

MET Antibody, AUTS9 Antibody, C-Met Antibody, HGF/SF receptor Antibody, HGFR Antibody, Met kinase Antibody, Proto-oncogene c-Met Antibody, Scatter factor receptor Antibody, SF receptor Antibody, HGF receptor Antibody, RCCP2 Antibody, Tyrosine-protein kinase Met Antibody

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules.

Western blot

Western blot
Anti-c-Met pY1349pY1356 Antibody - Western Blot. Western blot of affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary epithelial cells (B5/589) were serum deprived and stimulated with (+) and without (-) HGF. Cell lysates were immunoprecipitated using a human anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane and probed using various antibodies. Lane 1 and 2 where probed using the anti-c-Met pY1349pY1356 antibody (#LS-C60102), lane 3 and 4 where probed using an anti-phosphotyrosine antibody as phosphorylation control and lane 5 and 6 where probed using an anti-cMet antibody as a total Met loading control. Bands recognized at an apparent molecular weight of ~170 kD are total Met, ~145 kD phosphorylated Met beta chain in HGF +, ~150 kD phosphorylated Met in +/- HGF, ~50kD phosphorylated Met alpha chain. Personal communication with D. Bottaro and F. Cecchi, CCR-NCI, Bethesda, MD.

Western blot

Western blot
Anti-c-Met pY1349pY1356 Antibody - Western Blot. Western blot of affinity purified anti-c-Met pY1349pY1356 antibody shows detection of phosphorylated c-Met. Human mammary B5/589 epithelial cells were serum-deprived and treated with or without HGF. Cell lysates were immunoprecipitated with the anti-c-Met antibody, resolved by SDS-PAGE, transferred to PVDF membrane, and probed with anti-c-Met pY1349pY1356. Personal communication, D. Bottaro and T. Ito, NCI, Bethesda, MD.

Requested From: United States
Date Requested: 10/22/2016

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